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Page 1\nzeiss.com/axiocam\nZEISS Axiocam Family\nYour Guide to Microscope Camera Technology \nfrom ZEISS.\n\n\nPage 2\n2\n3\nCameras for Teaching or \nRoutine Applications\nHigh End Color \nCameras\nPolarization Camera\nHigh End Fluorescence \nCameras\nIntegrated Network \nCameras\n\n\nPage 3\nCameras for Teaching or Routine Applications 11\n10 Cameras for Teaching or Routine Applications\nAxiocam 105 color is your small, no-frills microscope camera. \nWith its compact design, it makes quick and efficient work of \nyour daily documentation needs.\nWith its USB 3.0 connection, experience a high speed data \ntransfer rate for handling your high resolution 5 megapixel \ncolor images. Offering an exposure time range of 30 µs to \n1 s and a live frame rate of up to 30 images per second, the \ncamera allows you to be well prepared to cover multiple \ntasks. Document your results quickly and conveniently.\nWith its attractive price-performance ratio, you can also \nexpand the capability of your fluorescence imaging system \nwith color imaging. Axiocam 105 color is ideal as a secondary \ncamera on fluorescence microscopes that are traditionally \nequipped with monochrome cameras.\nThe camera’s small form factor also lends itself well to \nenvironments with limited space.\nSimpler. More Intelligent. More Integrated.\n•\t 5 megapixel CMOS sensor\n•\t 30 images per second at full 5 megapixel color resolution\n•\t 8 bit digitization\n•\t High resolution with 2.2 µm pixel\n•\t Easy to use super-speed USB 3.0 connection\n•\t Color and black & white imaging modes\n•\t Fast and efficient operation with ZEN imaging software\nRecommended for\n•\t Applications with bright samples\n•\t Documentation\n•\t Education / Teaching\n•\t Routine tasks\n•\t Materials testing\n•\t Quality assurance / Quality control\nZEISS Axiocam 105 color\nYour 5 Megapixel Microscope Camera \nfor Documentation in Routine Labs\nFish, HE staining, brightfield, acquired with ZEISS Stemi 305\nGraphite in brightfield, objective: EC Epiplan-NEOFLUAR 20×\n0.0\n0.2\n0.4\n0.6\n0.8\n1.0\n400\n450\n500\n550\n600\n650\n700\n750\nRelative spectral sensitivity\nRelative Spectral Sensitivity\nWavelength (nm)\n\n\nPage 4\nCameras for Teaching or Routine Applications 13\n12 Cameras for Teaching or Routine Applications\n0.0\n0.1\n0.2\n0.3\n0.4\n0.5\n0.6\n0.7\n0.8\n0.9\n1.0\n400 450 500 550 600 650 700 750\n \n \nThis 5 megapixel CMOS camera from ZEISS offers you flexible \ntechnology at an impressive price-performance ratio. \nWith Axiocam ERc 5s you capture the finest details of \nstructures in your sample in high resolution. Connect your \ncamera in a multitude of ways. Attach it to your PC to acquire \ncrisp images with the easy-to-use imaging app Labscope \nor ZEN or use it as a digital video camera by connecting it \ndirectly to a monitor. Produce an exceptional live image for \nobserving dynamic processes. Or exploit the full flexibility of \nAxiocam ERc 5s as a standalone camera in your lab. You can \nstore your images directly onto an SD card and transfer them \nto a computer later, making your laboratory processes even \nmore efficient.\nThe camera can also be connected to a network via Ethernet \ncable. Wirelessly connect your iPad on the same network and \naccess the controls via Labscope. This means you can check \nin on your sample away from the system, freeing you to work \non other tasks. Or share the live image by connecting multiple \niPads to promote discussion amongst students or colleagues.\nSimpler. More Intelligent. More Integrated.\n•\t 5 megapixel CMOS sensor\n•\t 8 bit digitization\n•\t High resolution with 2.2 µm pixel\n•\t Color and black & white imaging modes\n•\t Flexibility – use Axiocam ERc 5s as a stand-alone camera \nand acquire images / movies directly to an SD card. \nOr use it as digital video camera and stream HD (720p60 or \n1080p30) via HDMI cable directly to a monitor or projector. \nYou control the camera via remote control and define your \nacquisition settings and store for later use to carry out \nroutine tasks independent of any computer. Alternatively \nuse it via Labscope with a network connection and an iPad\n•\t Efficient operation with Labscope or ZEN via an easy to \ninstall USB connection to a PC or via iPad / iPhone and \nLabscope App\nRecommended for\n•\t Applications with bright samples\n•\t Documentation\n•\t Education / Teaching\n•\t Routine tasks\n•\t Industrial work\n•\t Quality assurance / Quality control\nZEISS Axiocam ERc 5s\nYour 5 Megapixel Standalone Microscope Camera\nMicrasterias radiata (algae), brightfield\nPig gut, May-Grünwald-Giemsa staining\nRelative spectral sensitivity\nRelative Spectral Sensitivity\nWavelength (nm)\n\n\nPage 5\nCameras for Teaching or Routine Applications 15\n14 Cameras for Teaching or Routine Applications\nZEISS Axiocam 202 mono\nYour 2 Megapixel Stand-alone Microscope Camera \nfor Routine Fluorescence Documentation\nAxiocam 202 mono is your 2 megapixel monochrome \nmicroscope camera with automatic functions for routine \nfluorescence applications. With this CMOS sensor camera \nyou can easily acquire monochrome images in stand-alone \nmode with no need of a PC. Since the camera automatically \nadjusts the exposure time you only need to press the snap \nbutton to capture and store your fluorescence images on a \nUSB flash drive. If needed you can adjust parameters in the \nOSD (on screen display) menu before you acquire the image. \nIn combination with the smart microscopes Axiolab 5 or \nAxioscope 5 you can even capture multichannel fluorescence \nimages by simply pressing one button.\nRecommended for\n•\t Applications with bright fluorescence samples\n•\t Documentation\n•\t Education / Teaching\n•\t Fluorescence imaging applications with live and fixed cells\n•\t Documentation of fluorescent cell cultures\n•\t Routine tasks in cell laboratories\nSimpler. More Intelligent. More Integrated.\n•\t 2 megapixel CMOS chip sensor with image diagonal \nof 13 mm and large pixel size for high sensitivity in \nfluorescence documentation\n•\t Choose between 12 bit or 8 bit digitization\n•\t Store images directly on USB flash drive in stand-alone \nmode\n•\t Single button multichannel fluorescence acquisition when \ncombined with Axiolab 5 or Axioscope 5 stands in stand-\nalone mode (with no PC)\n•\t Automatic exposure and gain adjustment for easy \nfluorescence image capture\n•\t Connect directly to a monitor by a HDMI cable for live \nimage display for search and focussing and review of \nacquired images\n•\t Perform secure image data transfer to TWAIN-compatible \n3rd party software solutions with the TWAIN driver.\nMink endometrium cells, Vimentin (Ms) – Alexa Fluor 568, Phalloidin – Alexa \nFluor 488, Hoechst 33342, acquired with ZEISS Axioscope 5, objective: Plan-\nAPOCHROMAT 20× / 0.8\nIndian muntiac, deer epidermis fibroblasts, Tubulin (Ms) – Alexa Fluor 405, \nPhalloidin – Texas Red, SYTOX Green, acquired with ZEISS Axioscope 5, \nobjective: Plan-NEOFLUAR 10× / 0.3\n0.0\n0.1\n0.2\n0.3\n0.4\n0.5\n0.6\n0.7\n0.8\n0.9\n1.0\n400\n500\n600\n700\n800\n900\n1000\nRelative spectral sensitivity\nRelative Spectral Sensitivity\nWavelength (nm)\n\n\nPage 6\nCameras for Teaching or Routine Applications 17\n16 Cameras for Teaching or Routine Applications\nZEISS Axiocam 208 color\nYour Fast, 4K Microscope Camera \nfor Smart Digital Documentation\nAxiocam 208 color is your smart versatile 8 megapixel color \nmicroscope camera suitable for education, documentation \nand routine applications. This CMOS camera delivers crisp, \ndetail rich live images with high color fidelity at full 4k \nresolution in outstanding 30 fps. Choose between three \nmodes of operation:\n1.\t In stand-alone mode, you don’t need a PC to acquire \nmicroscope images. The camera automatically adjusts \nbrightness and white balance and offers live image \nenhancement functions like sharpening, denoising and \nHDR. Digital documentation of your specimen has never \nbeen easier.\n2.\t Alternatively, connect the CMOS camera via USB or to \na network and control it wirelessly with the easy-to-use \nimaging app Labscope. Since you can connect multiple \ncameras to the network, Axiocam 208 color is the \nideal solution for digital classroom applications and for \nconnected laboratories, too.\n3.\t In addition, you can use the powerful imaging software \nZEN with your Axiocam 208 color.\nRecommended for\n•\t Documentation\n•\t Education / Teaching\n•\t Routine tasks\n•\t Materials research\n•\t Quality assurance / Quality control\n•\t Fast high resolution live image for co-observation\nSimpler. More Intelligent. More Integrated.\n•\t Full 4K resolution in outstanding 30 fps\n•\t Brilliant color rendering\n•\t Live image enhancement functions like sharpening, \ndenoising and HDR\n•\t Use in stand-alone mode and save images on USB flash \ndrive, use Labscope imaging app or ZEN imaging software\n•\t Easy and effortless digital documentation – especially \nsuitable for education, digital classroom and routine \ndocumentation\n•\t Ethernet or USB 3.0 as digital data interface\n•\t Use the optional WiFi stick and Labscope imaging app to \ncontrol and transfer data wirelessly\n•\t Document your samples as you see it in the eyepieces\n•\t Stand-alone operation with camera control by intuitive \nOn Screen Display via mouse and keyboard without a PC\n•\t Connect directly to a monitor by a HDMI cable for live \nimage display for search and focussing and review of \nacquired images\n•\t Perform secure image data transfer to TWAIN-compatible \n3rd party software solutions with the TWAIN driver.\nTrichrome stained blood vessels in transmitted light brightfield, acquired with \nZEISS Axiolab 5, objective: Plan-APOCHROMAT 40× / 1.4\nRed bone marrow in transmitted light brightfield, acquired with ZEISS Axiolab 5, \nobjective: Plan-APOCHROMAT 40× / 1.4\n0.0\n0.1\n0.2\n0.3\n0.4\n0.5\n0.6\n0.7\n0.8\n0.9\n1.0\n400\n450\n500\n550\n600\n650\n700\nRelative spectral sensitivity\nRelative Spectral Sensitivity\nWavelength (nm)\n\n\nPage 7\nCameras for Teaching or Routine Applications 21\n20 Cameras for Teaching or Routine Applications\nAxiocam 305 color is your 5 megapixel camera for high \nresolution imaging at fast speeds. With state-of-the-art \nCMOS global shutter technology, you can follow and capture \nsamples distortion-free and with great accuracy. Thanks to \nthis highly sensitive sensor technology and precise camera \nengineering, your Axiocam 305 color allows the capture of \nquality color images for a wide range of applications. Acquire \ngreat color images with crisp contrast or use the optional \nblack & white mode to document basic fluorescence.\nWith this fast camera offering up to 36 frames per second at \nfull resolution, achieve efficient searching, fast focusing and \nergonomic handling at your digital microscope workplace. \nCover more of your area of interest with its 2/3″ sensor format \nand produce great color images on your compound, stereo, \nor zoom microscope.\nThough a simple and fast USB 3.0 connection, control the \ncamera and experience robust performance with easy to use \nZEN imaging software and its intuitive user interface.\nRecommended for\n•\t Applications with bright samples\n•\t Documentation\n•\t Routine tasks\n•\t Materials research\n•\t Quality assurance / Quality control\n•\t Fast high resolution live image for co-observation\n•\t Fast image acquisition and time-lapse recording\nZEISS Axiocam 305 color\nYour Fast 5 Megapixel Microscope Camera \nfor Routine and Research Labs\nSimpler. More Intelligent. More Integrated.\n•\t 5 megapixel CMOS global shutter sensor\n•\t 11.1 mm image diagonal\n•\t Fast readout with 36 images per second in full color \nresolution\n•\t 12 bit digitization for finer gradation in signal\n•\t Small 3.45 micron pixels for better sampling at low \nmagnifications\n•\t Global shutter architecture for distortion-free images\n•\t Active thermal stabilization of the sensor for extremely \nreproducible image quality\n•\t Easy to use super-speed USB 3.0 connection\n•\t Color and black & white imaging modes\n•\t Fast and efficient operation with ZEN imaging software\nLiver of Amphiuma in brightfield, HE-staining, acquired with ZEISS Axio \nImager, objective: EC Plan-NEOFLUAR 20× / 0.50\nPure iron in brightfield, reflected light, acquired with ZEISS Axio Observer, \nobjective: EC Epiplan-APOCHROMAT 50× / 0.9\n0.0\n0.1\n0.2\n0.3\n0.4\n0.5\n0.6\n0.7\n0.8\n0.9\n1.0\n400\n450\n500\n550\n600\n650\n700\nRelative spectral sensitivity\nRelative Spectral Sensitivity\nWavelength (nm)\n\n\nPage 8\nHigh End Color Cameras 27\n26 High End Color Cameras\nZEISS Axiocam 506 color\nYour 6 Megapixel Microscope Camera \nfor Fast Imaging in True Color\nThis high quality 6 megapixel color camera offers you an \namazingly fast live image and acquisition speed despite its \nlarge pixel count and the very large 16 mm diagonal field of \nview.\nThis makes it the camera of choice whenever large sample \nareas have to be screened and recorded by taking many \nimage tiles repeatedly in a minimum amount of time due to \nreduced number of tile positions.\nThis is extremely beneficial in acquiring large pathological \ntissue sections or large colored materials samples.\nRecommended for\n•\t Color imaging applications in life sciences and \nmaterials science\n•\t Co-observation with fast high resolution live image in \nhigh quality color with a very large field of view\n•\t Large pathology, cytology and materials samples\n•\t Fast tile scanning applications\n•\t Broadest range of intensities and exposure times\nHuman intestinal polyps, HE staining, acquired with ZEISS Axio Imager, \nobjective: Plan-APOCHROMAT 20× / 0.8\nHuman kidney, Azan staining, acquired with ZEISS Axio Imager, \nobjective: Plan-APOCHROMAT 10× / 0.45\nSimpler. More Intelligent. More Integrated.\n•\t 6 megapixel CCD sensor with 16 mm image diagonal\n•\t 19 full resolution color images per second\n•\t High image contrast with 14 bit signal conversion\n•\t Small 4.54 micron pixels for optimal resolution\n•\t Fast quad-port read-out with global shutter architecture \nfor distortion-free images\n•\t Black & white imaging mode\n•\t Reproducible image quality due to active thermal \nstabilization of the sensor\n•\t Easy to use super-speed USB 3.0 connection\n•\t Thermo electrical cooled sensor\n400\n450\n500\n550\n600\n650\n700\n0.0\n0.1\n0.2\n0.3\n0.4\n0.5\n0.6\n0.7\n0.8\n0.9\n1.0\nRelative spectral sensitivity\nWavelength (nm)\nRelative Spectral Sensitivity\n\n\nPage 9\nHigh End Color Cameras 29\n28 High End Color Cameras\nZEISS Axiocam 705 color\nYour Fast 5 Megapixel Microscope Camera \nfor True Color Image Acquisition in High Resolution\nThis flexible 5 megapixel scientific color camera strikes the \nperfect balance of speed and resolution. Delivering more \nthan 60 frames per second at full 5 megapixel resolution, this \ncamera captures even the most dynamic processes without \ncompromising image resolution. Subsampling or sub-region \nreadout accelerate your acquisition speed to hundreds of \nframes per second.\nRecommended for\n•\t High-resolution microscopy\n•\t High-framerate imaging\n•\t Research\n•\t Documentation\n•\t Industrial applications\n•\t Materials research\n•\t Quality control\n•\t Medical microscopy\n•\t Pathology\n•\t Cytology\nRat kidney section, objective: Plan-APOCHROMAT 40×/1.4 oil\nRat embryonic tissue section, objective: Plan-APOCHROMAT 63×/1.4 oil\nSimpler. More Intelligent. More Integrated.\n•\t 5 megapixel cooled color CMOS sensor \nwith 11 mm diagonal\n•\t 62 frames per second in full 5 megapixel resolution\n•\t Best-in-class color rendition\n•\t Color and monochrome imaging modes\n•\t Exclusive noise inhibition technology for low-light imaging\n•\t Dynamic range of 1:25,000 in high-dynamic range (HDR) \nmode\n•\t Combined analogue and digital pixel binning\n•\t Small 3.45 µm pixels for high-resolution imaging\n•\t Hardware triggering\n0\n0,1\n0,2\n0,3\n0,4\n0,5\n0,6\n0,7\n0,8\n0,9\n1\n400\n450\n500\n550\n600\n650\n700\nRelative Spectral Sensitivity\nWavelength (nm)\nRelative spectral sensitivity\n\n\nPage 10\nHigh End Color Cameras 31\n30 High End Color Cameras\nZEISS Axiocam 712 color\nYour All-round 12 Megapixel Microscope Camera for True Color \nAcquisition of Large Specimen Areas in High Resolution\nThis 12 megapixel scientific grade camera combines a large \nimage sensor, small pixel size, precise color rendition and \nfast imaging speed. The CMOS sensor delivers more than \n20 frames per second with a 17.5 mm diagonal large field of \nview. You can now acquire large specimen regions quickly \nand with uncompromised image quality. The large field of \nview reduces the number of tiles required to image largest \nsamples, and so drastically accelerates tiling experiments.\nAxiocam 712 color is a highly evolved digital color camera \naddressing the needs of scientific microscopy, including \ndocumentation, reporting and analysis. Fast and artifact-free \nimaging with optimized color reproduction makes your work \ncomfortable and efficient. In addition, exploring your sample \non the screen, instead through the oculars, becomes a true \nand very convenient alternative.\nRecommended for\n•\t High-resolution microscopy\n•\t Large region imaging\n•\t Medical imaging\n•\t Material science research\n•\t Macroscopic imaging\n•\t Pathology\nMouth region of a mouse embryo section, objective: Plan-APOCHROMAT \n63×/1.4 oil\nRat kidney section, objective: Plan-APOCHROMAT 63×/1.4 oil\nSimpler. More Intelligent. More Integrated.\n•\t 12 megapixel cooled color CMOS sensor\n•\t Large sensor with 17.5 mm diagonal for extended \nfield of view\n•\t Best-in-class color rendition\n•\t Color and monochrome imaging modes\n•\t 20 frames per second in full 12 megapixel resolution\n•\t 30 frames per second of the entire field of view in live \nimage mode\n•\t Exclusive noise inhibition technology for lowlight imaging\n•\t Dynamic range of 1:25,000 in high-dynamic range (HDR) \nmode\n0.0\n0.1\n0.2\n0.3\n0.4\n0.5\n0.6\n0.7\n0.8\n0.9\n1.0\n400\n450\n500\n550\n600\n650\n700\nRelative Spectral Sensitivity\nWavelength (nm)\nRelative spectral sensitivity\n\n\nPage 11\nHigh End Color Cameras 33\n32 High End Color Cameras\nZEISS Axiocam 807 color\nYour Fast, 7 Megapixel Microscope Camera for True Color \nImaging of Large Fields of View\nWith its dual USB interface and state-of-the art CMOS sensor, \nthis 7 megapixel color camera offers you amazingly fast live \nimage and acquisition speeds. The large 17.6 mm diagonal \nsensor allows for efficient imaging of large fields of view. \nThis makes it the camera of choice when screening large \nsample areas as it efficiently reduces the required number of \ntile images. This is extremely beneficial when working with \nlarge, pathology tissue sections or large, colored materials \nsamples.\nRecommended for\n•\t Color imaging applications in a broad range of fields from \nlife sciences to materials research and geoscience\n•\t Co-observation with fast high resolution live image in high \nquality color with a very large field of view\n•\t Imaging of large pathology, cytology and materials samples \n•\t Fast tile scanning applications\n•\t Samples with a broad range of intensities and exposure \ntimes\nPrussian blue staining for hemosiderin (iron deposition) in brown adipose \ntissue of mice. Sample courtesy: A. Feuchtinger, Helmholtz Zentrum München, \nGermany\nNASH mouse liver with connective collagenous tissue stained in red. \nSample courtesy: A. Feuchtinger, Helmholtz Zentrum München, Germany\nSimpler. More Intelligent. More Integrated.\n•\t 7 megapixel CMOS sensor with 17.6 mm image diagonal\n•\t 73 full-resolution color images per second recording \n(faster with sensor sub-region readout)\n•\t High image contrast with 14-bit signal conversion\n•\t 4.5 micron pixels for optimal resolution and sensitivity\n•\t Fast read-out with global shutter architecture for \ndistortion-free images\n•\t Monochrome imaging mode\n•\t Reproducible image quality due to active thermal \nstabilization of the sensor\n•\t Easy to use high-speed dual USB 3.0 connection\n0\n0.1\n0.2\n0.3\n0.4\n0.5\n0.6\n0.7\n0.8\n0.9\n1\n400\n410\n420\n430\n440\n450\n460\n470\n480\n490\n500\n510\n520\n530\n540\n550\n560\n570\n580\n590\n600\n610\n620\n630\n640\n650\n660\n670\n680\n690\n700\n710\n720\nRelative Spectral Responsivity\nWavelength (nm)\nRelative spectral responsivity\n\n\nPage 12\nHigh End Color Cameras 35\n34 High End Color Cameras\nZEISS Axiocam 820 color\nYour Sensitive 20 Megapixel Microscope Camera for Demanding, \nTrue Color Imaging of Large Fields of View\nAxiocam 820 color 20 megapixel camera combines high \nsensor resolution, color accuracy and a large sensor size for \nthe most challenging samples. Its live image is 30 frames per \nsecond, providing navigation with the PC that is as smooth as \nlooking through the eye pieces. The small pixels allow the use \nof low magnifications for high speeds and large fields of view \nwithout losing resolution. \nScan large areas faster than ever before due to its large \n17.5 mm square sensor. This makes Axiocam 820 color \nthe ideal choice for demanding color imaging in cytology, \npathology, materials research, and life science applications.\nRecommended for\n•\t The most demanding color applications in life sciences, \nmaterials research and geoscience \n•\t Co-observation with a fast, high-resolution live image in \nhigh quality color with a very large field of view\n•\t Large sample area imaging in pathology, cytology and \nmaterials samples \n•\t Fast tile scanning applications\n•\t The broadest range of intensities by high dynamic range \nMouse intestinal goblet cells with neutral (red) and acid (blue) mucine staining imaged with 20x/0.5 (left) and 63x/1.2W (right) objective. \nSample courtesy: A. Feuchtinger, Helmholtz Zentrum München, Germany\nSimpler. More Intelligent. More Integrated.\n•\t 20 megapixel square CMOS sensor with 17.5 mm image \ndiagonal\n•\t 28 full-resolution color images per second \n(faster with sensor sub-region readout)\n•\t Small 2.74 micron pixels for resolving the finest details \nat all magnifications\n•\t High-quality noise inhibition technology\n•\t Fast read-out with global shutter architecture for \ndistortion-free images\n•\t Best-in-class color rendition\n•\t Color and monochrome imaging modes\n•\t Reproducible image quality due to active thermal \nstabilization of the sensor\n•\t Dynamic range of 1:25,000 in high-dynamic range \n(HDR) mode\n0\n0.1\n0.2\n0.3\n0.4\n0.5\n0.6\n0.7\n0.8\n0.9\n1\n400\n410\n420\n430\n440\n450\n460\n470\n480\n490\n500\n510\n520\n530\n540\n550\n560\n570\n580\n590\n600\n610\n620\n630\n640\n650\n660\n670\n680\n690\n700\n710\n720\nNormalized Spectral Responsitivity\nWavelength (nm)\nNormalized spectral responsitivity\n\n\nPage 13\nKnowledge Base 69\n68 Knowledge Base\nSensor Size vs Camera Adapter vs Field of View (FOV)\nUse a c-mount camera adapter to mount your camera onto \nyour microscope. Depending on the magnification factor \nof the adapter, the camera’s sensor may cover more (lower \nmagnification) or less (higher magnification) of the image \ncoming out of your microscope (intermediate image). Typical \nintermediate image sizes are 25 mm for Axio Imager, 23 mm \nfor Axio Observer or 23 mm for Axio Zoom.V16.\nTypical image sensor diameters are 7.9 mm (1/2″ format), \n11 mm (2/3″ format) or 16 mm (1″ format).\nDifferent C-mount adapter magnifications are 1×, 0.63×, 0.5×.\nUsing a lower adapter magnification such as 0.63× causes into a:\n•\t Demagnification of the intermediate image, resulting in a \nlarger field of view for the final image\n•\t Enlargement of pixel size, thus increasing light intensity \ndetected by the sensor\n•\t Enlargement of pixel size, which reduces the effective \ncamera resolution\nResolution\nThe spatial resolution of a digital camera is related to the pixel \ndensity, which is defined by the pixel count per sensor area. \nThe smaller the pixel aperture, the finer is the sampling of the \npresented structure. The reproduction of fine structures (lines) \nrequires at least two pixels per structure sequence (line pair).\nDepending on the spectral composition of the signal, the \noptical resolution of color cameras can be slightly lower \ncompared to monochrome cameras because of the color filter \narray. However, elaborate interpolation algorithms allow color \ncameras to provide optimal image quality.\nPixel Size\n1 × 1\n2 × 2\n5 × 5\n480i\n640 × 480 pixels\n(66 % of 720p)\n720p\n1280 × 720 pixels\n(66 % of 1080p)\n1080p\n1920 × 1080 pixels\n10 × 10\n20 × 20\n50 × 50\n100 × 100\nThe pixel size defines the resolution.\nOne pixel is the smallest effective area on the sensor which is \nto become one image picture element.\nDifferent sensor sizes in relation to field of view.\nAdapter 1.0×\nAdapter 0.63×\nD = 23 mm\nD = 23 mm\nAxiocam 702\nAxiocam 702\nAxiocam 506\nAxiocam 506\nAxiocam 705\nAxiocam 705\nThe unit cell size can be estimated by taking the geometrical \nlength (height) of one sensor line (column) and dividing it by \nthe number of all pixels in one line (column).\nEffects of pixel size:\nSmaller pixels are\n•\t good for higher resolution\n•\t lower in dynamic range\n•\t less light sensitive\n•\t noisier\nLarger pixels are:\n•\t good for better light sensitivity\n•\t less noisy\n•\t higher in dynamic range\n•\t reducing the spatial resolution\nThe best pixel size is a balance between sensitivity (larger \npixel) and resolution (smaller pixel) to get the best possible \ncompromise for the imaging requirements at a given optical \nsetup.\nColor filter\nSensor pixel\nSensor pixel\nMonochrome and color sensors – a comparison.\nSame pixel count\n1 Pixel\n1 Pixel\nEven when the pixel count is the same, the image taken with the larger-sized \npixels is less noisy because the CCD sensor is larger.\nName of Effect\nRelated Limitation\nCounter Measure\nDark current\nSpurious signal by thermally generated electrons \ninside the sensor silicon material.\nThis signal varies from pixel to pixel and causes an \nexposure time dependent signal offset for each \nindividual pixel.\nIn addition it contributes to the signal noise.\nMaximum exposure time,\nLow light sensitivity, dynamic range, single pixel \ndefects (hot pixels)\nGiven for a specific sensor-technology, \nactive thermo-electrical cooling\nReadout noise\nNoise added to the signal during read-out\nLow light sensitivity, directly limiting the \npotential low light detection threshold, dynamic \nrange\nSensor design and analog signal management \ndependent, signal amplification by EMCCD \narchitecture\nPhoton shot noise\nPhysical property of light, proportional to square \nroot of produced electrons\nDetection precision at high intensity levels, \nnoisy, low light images\nTheoretical and practical limit of detection is \nabsolute, therefore no direct countermeasures\nADC effects\nDifferential and integral linearity effects, \nquantitation errors of Analog to Digital Converters\nDetection precision, intensity errors\nUse of good ADCs, use more bits than needed, \nsoftware calibration algorithms\nStatic sensor artefacts\nDefective pixels, non-uniformity effects of photo \nresponse, dark current, dark offset, electronic \nglow, hot pixels, column or row offsets, \nblack offset non-uniformities\nVisible cosmetic defects, fixed patterns in image \noverlaying image information\nOn the fly processing of the image data with \ncorrection algorithms, black reference, pixel wise \ndark current maps, use of selected sensors,\nCorrection by calibration of static effects, dead pixel \nstorage memory in camera\nDynamic sensor artefacts\nBlinking pixels, hot pixels, pixel and line offset \nflicker effects, electro-magnetic crosstalk of high \nfrequency interference effects, etc.\nVisible cosmetic defects, traveling overlaid \npatterns in image, subsequent artefacts in multi \nchannels or Z-stack images causing errors in 3D \nrenderings, errors in post processing algorithms \nlike segmentation, counting, etc.\nHigh quality electronic design, electronic shielding, \nhigh quality cables and connectors, on the fly \ndynamic correction algorithms, selection of high \nquality components, high quality sensors and dark \ncurrent calibration.\nOverview\nAdapter 0.63×\nAdapter 1.0×\nAxiocam 705\n2/3” Sensor\n11 mm diagonal\nPixelsize = 3.45 µm\n11 mm\n60 mm²\n27.8 mm\n370 mm²\nAxiocam 712\n1.1" Sensor\n17.5 mm / 0.63 = 27.8 diagonal\n3.45 µm / 0.63 = 5.48 µm\n\u00076× larger FOV\n\u0003\n6× higher speed\n\n\nPage 14\nKnowledge Base 71\n70 Knowledge Base\nNoise\nNoise in a digital camera is a random fluctuation of the image \nsignal which causes a detection error. Noise can come from \nvarious physical sources and it limits the detection capability \nof a given camera. Post-processing algorithms can be used to \nminimize noise, but this sacrifices other image factors such as \nresolution.\nSensor Cooling\nCooling is used to minimize the thermal generation of \nelectrons (dark current) in the sensor silicon material and \nthe resulting dark current noise. You can reduce the dark \ncurrent by approximately a factor of two by lowering \nthe sensor temperature by 7 °C. Active thermo-electrical \ncooling prevents the sensor from being heated by the power \ndissipation inside the camera electronics.\nDescription\nExplanation\nAdvantage\nDisadvantage\nAnalog Gain\nAmplification of the analog \nvoltage signal at the output \nof an image sensor before the \nAnalog-Digital-Converter (ADC)\n•\t Increases the brightness impression of \nthe signal\n•\t Needed to optimally adapt the analog \nsignal output from the camera sensor \nto the input range of the Analog-Digital \nConverter (ADC) within the camera \nelectronics\nSpecial case: in the case of a bottleneck \nfrom the ADC input range analog gain \ncan be used as sensitivity improvement\n•\t Standard case: when the ADC can handle the \nfull signal amplitude of the sensor no sensitivity \nimprovement can be achieved by analog gain\n•\t Images look very noisy\n•\t Reduction of available intra-scene dynamic range\nEM-Gain\nElectron Multiplication-Gain.\nDedicated on-chip high-voltage \nacceleration stage\n•\t Compensation for read noise limitation \n real detection improvement of low \nlight image signals\n•\t In combination with back thinning \ntechnology and large pixels providing \nbest possible low light sensitivity\n•\t Image affected by new noise source \nrandom bright pixel events \nminimization of EM-gain required\n•\t Gain efficiency affected by ageing \nlimited durability of EM gain\n•\t Reduction of available intra-scene dynamic range\nDigital Gain\nMultiplication of the digital \npixel value by a numerical factor\n•\t Mathematical way to increase \nbrightness\n•\t Commonly used for adapting different \nintensities to display different \nfluorescence channels in a multichannel \nimage\n•\t No increase in detection sensitivity\n•\t Histogram representation affected \ngaps in the histogram data\n•\t Reduction of available intra-scene dynamic range\nWays to amplify signals in cameras\nposition [pixel]\n100 counts noise\n0\n0\n100\n200\n300\n400\nintensity [counts]\n1000\n2000\n3000\n4000\nposition [pixel]\nwithout noise\n0\n0\n100\n200\n300\n400\nintensity [counts]\n1000\n2000\n3000\n4000\nDark Noise (Thermal Noise): origin by thermal electrons in the CCD cooling \nabout 8 – 10° reduces dark rushing by factor two\nCooling requires a heat sink to dissipate power from the \nthermo-electrical cooler itself. Additional measures are \nneeded to prevent condensation from humidity on the \ncold sensor surface. Modern sensors show a vastly reduced \namount of dark current compared to devices from the past. \nExtremely low temperatures – say, –20 °C – are not always \nrequired. Cooling is still unavoidable for EMCCD cameras, \ndue to their specific working principle. All other camera \ntechnologies have a benefit by cooling only at long exposure \ntimes (after some 30s and more), when the low dark current \nsums up and gets disturbing again.\nBinning\nCamera sensitivity can be increased by combining photo \ngenerated signal charges from neighboring pixels during \nread-out. This also increases the camera frame rate. One \nside effect is the loss of image resolution. Binning factors \ncan range from 1 × 1 (no binning) up to multiple pixels such \nas 5 × 5. Multiple charge binning is mainly available for CCD \nsensors. Binning in CMOS camera sensors is traditionally done \nin the digital domain by adding neighboring pixel values, \nwhich gives no extra sensitivity.\nFrame Rate\nThe frame rate of a digital camera denotes the number of \nimages which can be delivered per second (fps = frames \nper seconds). Unlike TV cameras, scientific cameras are not \nlimited to standard video frame rates. Digital camera frame \nrates depend on various parameters:\nThermo electrical cooling helps to minimize dark current effects of CCD and \nCMOS image sensors.\nPeltier cooler (white plate), \nmounted on heat sink\nSensor \nSocket\nHeat sink for cooler, connected \nwith housing for dissipation\nExposure time\nshorter = faster:\nExposure time limits the absolute frame rate independent from all other technical factors. If the time to collect \nphotons lasts for 100 ms, the maximum achievable frame rate is 1 / 100 ms = 10 fps.\nSensor readout speed / clock speed\nhigher = faster:\nTotal time to readout: accumulate photon signal+ conversion into a digital signal + transmission to a PC. \nExposure and Readout correspond to a full cycle of an image acquisition.\nPixel count\nless = faster:\nThe more pixels, the longer the readout cycle, the slower the frame rate.\nThe interface bandwidth can become the bottleneck if the pixel count cannot be transferred within the sensor \nreadout time.\nSensor sub frame / region of \ninterest\nsmaller = faster:\nDefinition of sensor sub areas (ROI) help reduce the amount of transmitted image data \n frame rates can be increased,\nPrerequisite: exposure time is shorter then readout time of ROI\nBandwidth of digital interface\nhigher = faster:\nData transfer capacity of the interface. Effective USB 3.0 bandwidth is approximately 320 Mbytes / s.\nParallel readout architecture of \nCMOS sensors\nmore = faster:\nCMOS sensors exceed the frame rates of comparably sized CCD sensors due to significantly more parallel output \nstructures on the sensor.\nThe interface bandwidth is more likely to be the data transfer bottleneck.\nTrigger signal synchronization\nSynchronization of external trigger components with image acquisition \n reduction of the maximum achievable frame rates with improvement of precision.\nOverlapping readout and exposure\nSpecial optimization for fast time series acquisition (fast time-lapse) without switching external components \n overlap of exposure event while readout of the previous image.\nOnly if exposure time is longer than readout frame rate limited by exposure time.\n\n\nPage 15\nKnowledge Base 73\n72 Knowledge Base\nHot Pixel\nCosmetic sensor defects are caused by a local emission of \nelectrons in the sensor material. Hot pixels are visible as \nstatic single bright pixels against the black background. Their \nintensity varies widely and scales with exposure time and \nsensor temperature. The signal cannot be differentiated from \nphoton generated electrons. If the sensor is temperature \nstabilized, the dark current can be compensated for by \nsubtracting the spurious signal in correspondence with \nexposure time. Saturated pixels need to be interpolated \nbecause image information in these pixels is lost and cannot \nbe reconstructed. Cosmic radiation can induce new hot pixel \ndefects over time.\nSpectral Sensitivity / Quantum efficiency\nAll kind of light detectors show a wavelength dependent light \nsensitivity. The conversion efficiency is the ratio of incoming \nphotons to generated signal electrons stated as a percentage. \nDetection range of silicon based sensors like CCD or CMOS \ncan stretch from approximately 350 nm up to 1000 nm, with \na peak between 500 nm – 600 nm. For detection of radiation \noutside of this spectral range, other materials need to be \nused.\nModern front illuminated devices offer a typical QE in the \nrange of 70 %. Monochrome peak QE can be improved with \nback thinned technology by up to 95 % in peak.\nZEISS Axiocam 702 mono offers extended flexibility for long exposure times up to 60 s\nLeft: Dark background, non uniformity from common CMOS sensor at 10s, \nRight: ZEISS Axiocam 702 mono with modern CMOS sensor at 10s with very dark background, low non uniformity\nCMOS Sensor with high dark current, 10s, 4 °C\nZEISS Axiocam 702 mono, 10s, 15 °C\nCMOS sensor with long exposure times\nZEISS Axiocam 702 mono\nThe spectral sensitivity of color cameras is lower than \nmonochrome cameras. The color filter dyes on the pixels \nreduce the peak spectral QE by approximately 15 %. Color \ncameras also need an IR filter as color is only defined in the \nvisible spectrum.\nColor Temperature\nSunrise / Sunset\nCandlelight\n2,000 K\nIncandescent\nLight Bulb\n3,000 K\nDaylight / Flash\n5,000 K\nCloudy Day\n7,000 K\nShade Under\na Blue Sky\n10,000 K\nColor temperature is a temperature value (in Kelvin) of a light \nsource and is used to describe the spectral characteristic of \nthe corresponding spectral emission. It indicates the color \nimpression of a light source: lower temperatures are more \nred, higher temperatures are more blue.\nThe color temperature of the light influences how the human \neye perceives color.\nWhite Balance\nThe color of the illuminating light source influences the color \nof an object. The relative intensity of the color channels of a \ncolor camera needs to be adjusted to assure a neutral color \nreproduction. For this, you will need a manual or automatic \nselection of a neutral (grey) point in the image. Fine-tune the \ncolor reproduction by assigning slightly shifted target values \nfor the neutral point. Adjust the color temperature of the \nmonitor (i.e. 3,200 K) to reach the desired color reproduction.\nDisplay Curve\nThe image display curve is a powerful tool in ZEN imaging \nsoftware, used to define how image data is displayed on \na computer screen without changing the raw image data. \nUse this tool to adjust dark areas of your image visually by \nselectively changing the curvature or the steepness of the \ncurve. Shift the minimum or maximum points to allow for \nthe limitation of the visualized intensity range. The color \nrendition can be influenced by a Gamma curvature. Image \ncharacteristics are applied to the image data, if the image \ngets exported into non .CZI image formats.\nGamma adjustment – linear display\nGamma Adjustment – Gamma 0.45\n400\n450\n500\n550\n600\n650\n700\n0.0\n0.1\n0.2\n0.3\n0.4\n0.5\n0.6\n0.7\n0.8\n0.9\n1.0\nRelative spectral sensitivity ZEISS Axiocam 506 color.\nWavelength (nm)\nRelative Spectral Sensivity\n\n\nPage 16\nKnowledge Base 77\n76 Knowledge Base\nTechnical Data\nColor Cameras\nAxiocam ERc 5s\nAxiocam 105 color\nAxiocam 208 color\nAxiocam 305 color\nAxiocam 506 color\nAxiocam 705 color\nAxiocam 712 color\nSensor type\nCMOS, Rolling Shutter\nCMOS, Rolling Shutter\nCMOS, Rolling Shutter\nCMOS, Global Shutter\nCCD, Quad Port Progressive Scan\nCMOS, Global Shutter\nCMOS, Global Shutter\nSensor size\n5.7 mm × 4.28 mm\nequivalent 1/2.5″\ndiagonal 7.1 mm\n5.70 mm × 4.28 mm\nequivalent to 1/2.24″\ndiagonal 7.13 mm\n7.1 mm × 4.0 mm\nequivalent to 1/2.1″\ndiagonal 8.1 mm\n8.5 mm × 7.1 mm\nequivalent 2/3″\ndiagonal 11.1 mm\n12.2 mm × 9,8 mm\nequivalent to 1″\ndiagonal 16 mm\n8.5 mm × 7.1 mm\nequivalent to 2/3″\ndiagonal 11.1 mm\n14.1 mm × 10.4 mm\nequivalent to 1″\ndiagonal 17.5 mm\nPixel Count\n5.0 megapixel:\n2560 (H) × 1920 (V)\n5.0 megapixel:\n2592 (H) × 1944 (V)\n8.3 megapixel:\n3840 (H) × 2160 (V)\n5.07 megapixel:\n2464 (H) × 2056 (V)\n6 megapixel:\n2752 (H) × 2208 (V)\n5.07 megapixel:\n2464 (H) × 2056 (V)\n12 megapixel:\n4096 (H) × 3008 (V)\nSubsampling\n–\n–\n–\n1×1, 2×2\n–\n1×1, 2×2\n1×1, 2×2\nPixel size\n2.2 µm × 2.2 µm\n2.2 µm × 2.2 µm\n1.85 µm × 1.85 µm\n3.45 µm × 3.45 µm\n4.54 µm × 4.54 µm\n3.45 µm × 3.45 µm\n3.45 µm × 3.45 µm\nFull Well Capacity\n–\n–\n–\n10,500 e⁻\n15,000 e⁻\n11,000 e⁻\n11,000 e⁻\nSensor Filter Mask\nRGB Bayer Filter\nRGB Bayer Filter\nRGB Bayer Filter\nRGB Bayer Filter\nRGB Bayer Filter\nRGB Bayer Filter\nRGB Bayer Filter\nSpectral Sensitivity\nApprox. 400 nm – 700 nm, \nIR filter\nApprox. 400 nm – 670 nm, \nIR filter\nApprox. 400 nm – 700 nm, \nIR filter\nApprox. 380 nm – 720 nm, \ncoated IR cut filter\nApprox. 400 nm – 720 nm, \ncoated IR cut filter\nApprox. 400 nm – 720 nm, \ncoated IR cut filter\nApprox. 400 nm – 720 nm, \ncoated IR cut filter\nBinning\nDigital binning\nNo\nNo\nDigital binning \n1×, 2×, 3×, 4×, 5×\nCharge binning \n1×1, 2×2, 3×3, 4×4, 5×5\nDigital binning \n1×, 2×, 3×, 4×, 5×\nDigital binning \n1×1, 2×2, 3×3, 4×4, 5×5\nROI (Region of Interest)\nYes (adjustable)\nYes (adjustable)\nFixed frame 1080p mode\nYes (adjustable)\nYes (adjustable)\nYes (adjustable)\nYes (adjustable)\nReadout Noise\n–\n–\n–\nTyp. 2.2 e⁻ @ gain 1×\nTyp. < 6.5 e⁻ (39 Mhz),\nTyp. 6 e⁻ (13 Mhz)\nTyp. 2,2 e⁻ @ gain 1×,\nTyp. 1,15 e⁻ @ gain 16×\nTyp. 2,2 e⁻ @ gain1×,\nTyp. 1,15 e⁻ @ gain 16×\nDark current\n–\n–\n–\nTyp. < 1.0 e⁻/p / s @ 25°C\nTyp. < 0,06 e⁻/p / s at 18 °C\nTyp. < 0.5 e⁻/p / s @ 18 °C\nTyp. < 0.5 e⁻/p / s @ 18 °C\nDynamic range\n–\n–\n–\nTyp. 1:4800\nTyp. 1:2500\nTyp. 1:5000 at gain 1×, \n1:25,000 at HDR mode\nTyp. 1:5000 at gain 1×, \n1:25,000 at HDR mode\nDigitization Bit Depth\n8 bit\n8 bit\n8 bit\n12 bit / 8 bit\n3× 14 bit / 12 bit / 8 bit\n14 bit / 12 bit / 8 bit\n3× 14 bit / 12 bit / 8 bit\nExposure Time Range\n10 µs – 2 s\n30 µs – 1 s\n61 µs – 1 s\n100 µs – 4 s\n250 µs – 60 s\n100 µs to 60 s\n100 µs to 60 s\nAnalog Gain\nNo\nYes\n1× – 22× adjustable\n1×, 2×, 4×, 8×, 16×\n1×, 2×, 3×\n1×, 2×, 4×, 8×, 16×\n1×, 2×, 4×, 8×, 16×\nFrame rate live image / Time Lapse \nRecording\nLive 20 fps at 800 × 600,\nNot recommended for time lapse \nimaging\nLive 17 fps at 5 MP\nLive\nEthernet: 30 fps at \n4K/1080p (H.264)\nUSB 3.0: 30 fps at \n4K/1080p (MJPEG)\nHDMI: 30 fps at 4K/1080p\nNot recommended for timelapse \nimaging in ZEN\nLive 30 fps at 5 MP \n67 fps at 1920 × 1080\n(ROI in HD format)\n136 fps at 512 × 512 (ROI)\nLive at 19 fps at 6 MP\n19 fps at 2752 × 2208;\n32 (ROI in HD format)\n33 fps at 917 × 733;\n51 fps at 550 × 440\nLive 30 fps 5 MP\n60 fps at 2464 × 2056 (5MP)\n115 fps at 1920 × 1080 (HDTV format)\n436 fps at 1920 × 256\nLive 30 fps at 2048 × 1504,\nLive 20 fps at full frame\n23 fps full frame\n63 fps at 1920 × 1080 (HDTV)\nup to 430 fps at 1020 × 120\nSensor cooling\nNo\nNo\nNo\nstabilized at 25°C\nstabilized at 18°C\nstabilized at 18°C\nstabilized at 18°C\nExternal trigger\nNo\nNo\nNo\nNo\nYes\nYes\nYes\nInterface\n1× SD card slot,\n1× mini USB 2.0,\n1× RJ 45 (LAN),\n1× HDMI (DVI-D)\nUSB 3.0 Micro-B (Camera) to \nUSB 3.0 Standard A (PC / Board)\nUSB 3.0 Type C, Ethernet, HDMI, \npower\nUSB 3.0 SuperSpeed (5 Gbit / s); \nBandwidth max. 240 MB / s;\nUSB 2.0 optional, with lower speed;\nUSB 3.0 SuperSpeed (5 Gbit / s); \nBandwidth max. 240 MB / s;\nUSB 2.0 optional, with lower speed;\nUSB 3.0 SuperSpeed (5 Gbit / s); \nBandwidth max. 330 MB / s;\nUSB 2.0 optional, with lower speed;\nUSB 3.0 SuperSpeed (5 Gbit / s); \nBandwidth max. 330 MB / s;\nUSB 2.0 optional, with lower speed;\nPower consumption \nand supply\n5 W through 2× USB 2.0\nmax. 0.5 – 1 W through USB 3.0\n9 W, external power supply\n4W, powered by USB 3.0-Bus \nfrom PC\n7W, powered by USB 2.0 and \nUSB 3.0-Bus from PC;\n7W, powered by USB 2.0 and \nUSB 3.0-Bus from PC;\n7W, powered by USB 2.0 and \nUSB 3.0-Bus from PC;\nSoftware\nZEN blue, ZEN core, Labscope\nZEN blue, ZEN core\nZEN blue, ZEN core, Labscope\nZEN blue, ZEN core, Labscope\nZEN blue, ZEN core\nZEN blue, ZEN core\nZEN blue, ZEN core\n\n\nPage 17\nKnowledge Base 79\n78 Knowledge Base\nTechnical Data\nColor Cameras\nAxiocam 705 pol\nAxiocam 807 color\nAxiocam 820 color\nSensor type\nCMOS, Global Shutter\nCMOS, Global Shutter Gen3\nCMOS, Global Shutter, Back Iluminated\nSensor size\n8.5 mm × 7.1 mm\nequivalent to 2/3″\ndiagonal 11.1 mm\n14.5 mm × 9.9 mm \nequivalent to 1.1″ \ndiagonal 17.6 mm\n12.4 mm × 12.4 mm \n(square field of view), \nequivalent to 1.1″ \ndiagonal 17.5 mm\nPixel Count\n5.07 megapixel:\n2464 (H) × 2056 (V)\n7.1 megapixel: \n3216 (H) × 2208 (V)\n20 megapixel: \n4512 (H) × 4512(V)\nSubsampling\n1 × 1\n1 × 1, 2 × 2\n1 × 1, 2 × 2\nPixel size\n3.45 µm × 3.45 µm,\n6.9 µm effective pixel size based on \npolarization filter cell size\n4.5 µm × 4.5 µm\n2.74 µm × 2.74 µm\nFull Well Capacity\n11,000 e⁻\n25,000 e⁻\n10,000 e⁻\nSensor Filter Mask\nPolarization Filter (0°, 45°, 90°, 135°)\n78% @ 520 nm \n–\nSpectral Sensitivity\nApprox. 350 nm – 1,000 nm, \ncoated protective glass\nApprox. 400 nm – 720 nm, \ncoated IR Cut filter \nApprox. 400 nm – 720 nm, \ncoated IR Cut filter \nBinning\nDigital binning \n1×1\nHybrid binning \n1×, 2×, 3×, 4×, 5×\nHybrid binning \n1×, 2×, 3×, 4×, 5×\nROI (Region of Interest)\nYes (adjustable)\nYes (adjustable)\nYes (adjustable)\nReadout Noise\nTyp. 2.2 e⁻ @ gain 1×\nTyp. 1.15 e⁻ @ gain 16×\nTyp. 5.7 e⁻ @ gain 1× \ndown to 2.9 e⁻ @ gain 16×\nTyp. 2.3 e⁻ @ gain 1× \ndown to 1.3 e⁻ @ gain 16×\nDark current\nTyp. < 0.5 e⁻/p / s @ 18°C\nTyp. 0.3 e⁻/p / s @ 25°C\nTyp. < 0.1 e⁻/p / s @ 25°C\nDynamic range\nTyp. 1:5,000 at gain 1×, \n1:25,000 at HDR mode\nTyp. >1:4,420 at gain 1×, \nLow Noise Mode 1:6,230\nTyp. 1:4,400 at gain 1×, \nHDR 1:25,000 \nDigitization Bit Depth\n14 bit / 12 bit / 8 Bit\n3× 14 bit / 12 bit / 8 bit \n3× 14 bit / 12 bit / 8 bit \nExposure Time Range\n100 µs – 60 s\n0.1 ms to 60 s\n100 µs to 60 s\nAnalog Gain\n1×, 2×, 4×, 8×, 16×\n1×, 2×, 4×, 8×, 16×\n1×, 2×, 4×, 8×, 16×\nFrame rate live image / Time Lapse \nRecording\nLive 25 fps 5 MP (values only for \nmonochrome or fast color modes),\nLive 30 fps at 1920 × 1080\n60 fps at 2464 × 2056 (5MP)\n115 fps at 1920 × 1080 (HDTV \nformat)\n436 fps at 1920 × 256\nLive at 30 fps at 7 MP \n73 fps at 3216 × 2208 \n145 fps at 1920 × 1080 (HDTV) \n260 fps at 1608 × 1104 \n487 fps up to 1920 × 256\nLive 30 fps at 2256 × 2256 \n28 fps at 4512 × 4512 \n75 fps at 2256 × 2256 (subsampl.) \n110 fps at 1920 × 1080 (HDTV) \nUp to 447 fps at 1920 × 128\nSensor cooling\nstabilized at 18 °C\nstabilized at 25 °C\nstabilized at 25 °C\nExternal trigger\nYes\nYes \nYes \nInterface\nUSB 3.0 SuperSpeed (5 Gbit / s); \nBandwidth max. 330 MB / s;\nUSB 2.0 optional, with lower speed;\nDual USB 3.0 (2× 5 GBit/s); \nBandwidth max. 620 MB / s; \nSingle USB 3.0 operation \nat lower speed\nDual USB 3.0 (2× 5 GBit/s); \nBandwidth max. 620 MB / s; \nSingle USB 3.0 operation \nat lower speed\nPower consumption \nand supply\n7W, powered by USB 2.0 and \nUSB 3.0-Bus from PC;\n7W, powered by \nDual USB 3.0-Bus from PC, 5V\n7W, powered by \nDual USB 3.0-Bus from PC, 5V\nSoftware\nZEN blue, ZEN core\nZEN blue, ZEN core\nZEN blue, ZEN core\n\n\nPage 18\nKnowledge Base 81\n80 Knowledge Base\nTechnical Data\nMonochrome Cameras\nAxiocam 202 mono\nAxiocam 305 mono\nAxiocam 506 mono\nAxiocam 702 mono\nAxiocam 705 mono\nAxiocam 712 mono\nAxiocam 807 mono\nAxiocam 820 mono\nSensor type\nCMOS, Global Shutter\nCMOS, Global Shutter\nCCD, Quad Port Progressive Scan\nCMOS, Global Shutter\nCMOS, Global Shutter\nCMOS, Global Shutter\nCMOS, Global Shutter\nCMOS, Global Shutter, Back Iluminated\nSensor size\n11.25 mm × 6.33 mm\nequivalent to 1/1.2″\ndiagonal 13.4 mm\n8.5 mm × 7.1 mm\nequivalent 2/3″\ndiagonal 11.1 mm\n12.2 mm × 9.8 mm\nequivalent to 1″\ndiagonal 16 mm\n11.3 mm × 7.1 mm\nequivalent to 1/1.2″\ndiagonal 13.3 mm\n8.5 mm × 7.1 mm\nequivalent to 2/3″\ndiagonal 11.1 mm\n14.1 mm × 10.4 mm\nequivalent to 1″\ndiagonal 17.5 mm\n14.5 mm × 9.9 mm \nequivalent to 1.1″ \ndiagonal 17.6 mm\n12.4 mm × 12.4 mm \n(square field of view), \nequivalent to 1.1″ \ndiagonal 17.5 mm\nPixel Count\n2 megapixel: \n1920 (H) × 1080 (V)\n5.07 megapixel: \n2464 (H) × 2056 (V)\n6 megapixel: \n2752 (H) × 2208 (V)\n2.4 megapixel: \n1920 (H) × 1216 (V)\n5.07 megapixel: \n2464 (H) × 2056 (V)\n12 megapixel: \n4096 (H) × 3008 (V)\n7.1 megapixel: \n3216 (H) × 2208 (V)\n20 megapixel: \n4512 (H) × 4512(V)\nSubsampling\n–\n1 × 1, 2 × 2\n1 × 1, 2 × 2\n1 × 1, 2 × 2\n1 × 1, 2 × 2\n1 × 1, 2 × 2\nPixel size\n5.86 µm × 5.86 µm\n3.45 µm × 3.45 µm\n4.54 µm × 4.54 µm\n5.86 µm × 5.86 µm\n3.45 µm × 3.45 µm\n3.45 µm × 3.45 µm\n4.5 µm × 4.5 µm\n2.74 µm × 2.74 µm\nFull Well Capacity\n–\n10,500 e⁻\n15,000 e⁻\n32,000 e⁻\n11,000 e⁻\n11,000 e⁻\n25,000 e⁻\n10,000 e⁻\nQuantum Efficiency\n–\n69% @ 525 nm\n74% @ 500nm\n78% @ 525 nm\n72% @ 550 nm\n74% @ 500 nm\n78% @ 520 nm \n–\nSpectral Sensitivity\nApprox. 350 nm – 1,000 nm, \ncoated protective glass\nApprox. 380 nm – 1,000 nm, \ncoated protective glass\nApprox. 350 nm – 1,000 nm, \ncoated protective glass\nApprox. 350 nm – 1,000 nm, \ncoated protective glass\nApprox. 350 nm – 1,000 nm, \ncoated protective glass\nApprox. 350 nm – 1,000 nm, \ncoated protective glass\nApprox. 350 nm – 1,000 nm, \ncoated protective glass\nApprox. 350 nm – 1,000 nm, \ncoated protective glass \nBinning\nNo\nDigital binning \n1×, 2×, 3×, 4×, 5×\nCharge binning \n1 × 1, 2 × 2, 3 × 3, 4 × 4, 5 × 5\nDigital binning \n1 × 1, 2 × 2, 3 × 3, 4 × 4, 5 × 5\nDigital binning \n1×, 2×, 3×, 4×, 5×\nDigital binning \n1 × 1, 2 × 2, 3 × 3, 4 × 4, 5 × 5\nHybrid binning \n1×, 2×, 3×, 4×, 5×\nHybrid binning \n1×, 2×, 3×, 4×, 5×\nROI (Region of Interest)\nFixed frame 1080p mode\nYes (adjustable)\nYes (adjustable)\nYes (adjustable)\nYes (adjustable)\nYes (adjustable)\nYes (adjustable)\nYes (adjustable)\nReadout Noise\n–\nTyp. 2.2 e⁻ @ gain 1×\nTyp. < 6.5 e⁻ (39 Mhz),\nTyp. 6 e⁻ (13 Mhz)\nTyp. 6 e⁻ @ gain 1×\nTyp. 3.75 e⁻ @ gain 16×\nTyp. 2.2 e⁻ @ gain 1×\nTyp. 1.15 e⁻ @ gain 16×\nTyp. 2.2 e⁻ @ gain 1×\nTyp. 1.15 e⁻ @ gain 16×\nTyp. 5.7 e⁻ @ gain 1× \ndown to 2.9 e⁻ @ gain 16×\nTyp. 2.3 e⁻ @ gain 1× \ndown to 1.3 e⁻ @ gain 16×\nDark current\n–\nTyp. < 1.0 e / p/s @ 25°C\nTyp. < 0.06 e⁻/p / s at 18°C\n1.1 e⁻/p / s at 18 °C\nTyp. < 0.5 e⁻/p / s @ 18°C\nTyp. < 0.5 e⁻/p / s @ 18°C\nTyp. 0.3 e⁻/p / s @ 25°C\nTyp. < 0.1 e⁻/p / s @ 25°C\nDynamic range\n–\nTyp. 1:4,800\nTyp. 1:2,500\nTyp.> 1:5,000 at gain 1×\nHDR Mode 25,000:1\nTyp. 1:5,000 at gain 1×,\n1:25,000 at HDR mode\nTyp. 1:5,000 at gain 1×,\n1:25,000 at HDR mode\nTyp. >1:4,420 at gain 1×, \nLow Noise Mode 1:6,230\nTyp. 1:4,400 at gain 1×, \nHDR 1:25,000 \nDigitization Bit Depth\n8 and 12 bit\n12 bit / 8 bit\n14 bit / 12 bit / 8 bit\n14 bit / 12 bit / 8 bit\n14 bit / 12 bit / 8 Bit\n14 bit / 12 bit / 8 bit adjustable\n14 bit / 12 bit / 8 bit \n14 bit / 12 bit / 8 bit \nExposure Time Range\n61 µs – 2 s\n100 µs – 4 s\n250 µs – 60 s\n100 µs – 60 s\n100 µs – 60 s\n100 µs to 60 s\n0.1 ms to 60 s\n100 µs to 60 s\nAnalog Gain\n1× – 16× adjustable\n1×, 2×, 4×, 8×, 16×\n1×, 2×, 3×\n1×, 2×, 4×, 8×, 16×\n1×, 2×, 4×, 8×, 16×\n1×, 2×, 4×, 8×, 16×\n1×, 2×, 4×, 8×, 16×\n1×, 2×, 4×, 8×, 16×\nFrame rate live \nimage / Time Lapse \nRecording\nLive\nEthernet: 30 fps at 1080p \n(H.264)\nUSB 3.0: 30 fps at 1080p \n(MJPEG)\nHDMI: 30 fps at 1080p\nNot recommended for \nTimelapse imaging in ZEN\nLive 30 fps at 5 MP\n67 fps at 1920 × 1080\n(ROI in HD format)\n136 fps at 512 × 512 (ROI)\nLive 19 fps at 6 MP\n19 fps at 2752 × 2208;\n32 (ROI in HD format)\n33 fps at 917 × 733;\n51 fps at 550 × 440\nLive 30 fps at 2.4 MP\ntime lapse:\n128 fps at 1920 × 1216\n210 fps at 1929 × 720\n534 fps at 1920 × 128\n1,000 fps at 1024 × 128\nLive 30 fps 5 MP\n60 fps at 2464 × 2056 (5MP)\n115 fps at 1920 × 1080 (HDTV format)\n436 fps at 1920 × 256\nLive 30 fps at 2048 × 1504,\nLive 20 fps at full frame\n23 fps full frame\n63 fps at 1920 × 1080 (HDTV)\nup to 430 fps at 1020 × 120\nLive at 30 fps at 7 MP \n73 fps at 3216 × 2208 \n145 fps at 1920 × 1080 (HDTV) \n260 fps at 1608 × 1104 \n487 fps up to 1920 × 256\nLive 30 fps at 2256 × 2256 \n28 fps at 4512 × 4512 \n75 fps at 2256 × 2256 (subsampl.) \n110 fps at 1920 × 1080 (HDTV) \nUp to 447 fps at 1920 × 128\nSensor cooling\nNo\nstabilized at 25 °C\nstabilized at 18°C\nstabilized at 18°C\nstabilized at 18 °C\nstabilized at 18 °C\nstabilized at 25 °C\nstabilized at 25 °C\nExternal trigger\nNo\nNo\nYes\nYes\nYes\nYes\nYes \nYes \nInterface\nUSB 3.0 Type C,\nEthernet,\nHDMI, power\nUSB 3.0 SuperSpeed (5 Gbit / s); \nBandwidth max. 240 MB / s;\nUSB 2.0 optional, \nwith lower speed;\nUSB 3.0 SuperSpeed (5 Gbit / s);\nBandwidth max. 240 MB / s;\nUSB 2.0 optional, \nwith lower speed;\nUSB 3.0 SuperSpeed (5 \nGbit / s); \nBandwidth max. 240 MB / s;\nUSB 2.0 optional, \nwith lower speed\nUSB 3.0 SuperSpeed (5 Gbit / s); \nBandwidth max. 330 MB / s;\nUSB 2.0 optional, with lower speed\nUSB 3.0 SuperSpeed (5 Gbit / s); \nBandwidth max. 330 MB / s;\nUSB 2.0 optional, with lower speed\nDual USB 3.0 (2× 5 GBit/s); \nBandwidth max. 620 MB / s; \nSingle USB 3.0 operation \nat lower speed\nDual USB 3.0 (2× 5 GBit/s); \nBandwidth max. 620 MB / s; \nSingle USB 3.0 operation \nat lower speed\nPower consumption and \nsupply\n9W, external power supply\n4W, powered by USB 3.0-Bus \nfrom PC\n7W, powered by USB 2.0 and \nUSB 3.0-Bus from PC;\n7W, powered by USB 2.0 and \nUSB 3.0-Bus from PC;\n7W, powered by USB 2.0 and \nUSB 3.0-Bus from PC;\n7W, powered by USB 2.0 and \nUSB 3.0-Bus from PC;\n7W, powered by \nDual USB 3.0-Bus from PC, 5V\n7W, powered by \nDual USB 3.0-Bus from PC, 5V\nSoftware\nZEN blue, ZEN core, Labscope\nZEN blue, ZEN core, Labscope\nZEN blue, ZEN core\nZEN blue, ZEN core\nZEN blue, ZEN core\nZEN blue, ZEN core\nZEN blue, ZEN core\nZEN blue, ZEN core\n\n\nPage 19\nKnowledge Base 83\n82 Knowledge Base\nAxiocam ERc 5s\nAxiocam 105 color\nAxiocam 208 color\nAxiocam 305 color\nAxiocam 506 color\nAxiocam 705 color\nAxiocam 712 color\nAxiocam 705 pol\nAxiocam 807 color\nAxiocam 820 color\nHistology / Pathology\n++\n++\n+++\n+++\n++++\n++++\n++++\n+\n++++\n++++\nLive Cell Imaging\n+\n+\n+\n++\n+++\n+++\n+++\n++++\n+++\n+++\nFluorescence Imaging\n+\n+\n+\n++\n++\n++\n++\n++++\n++\n++\nLow Light Imaging for Dim Samples\n+\n+\n+\n++\n+++\n+++\n+++\n++++\n+++\n+++\nSemiconductor Inspection\n++\n++\n++\n+++\n++++\n++++\n++++\n++++\n++++\n++++\nLarge Samples\n+\n+\n+\n+++\n++++\n+++\n++++\n+++\n++++\n++++\nMaterials Research\n++\n+++\n+++\n++++\n++++\n++++\n++++\n++\n++++\n++++\nQuality Control\n+++\n+++\n++++\n+++\n+++\n+++\n++++\n+\n++++\n++++\nTeaching\n++++\n+++\n++++\n+++\n+\n+++\n+\n++\n+\n+\nClinical Routine\n+++\n++++\n++++\n+++\n+\n+++\n++\n+\n+\n++\nDynamic Range\n+\n+\n+++\n++++\n++++\n++++\n++++\n++++\n++++\n++++\nColor Rendition\n++\n+++\n++++\n++++\n++++\n++++\n++++\n++++\n++++\nPolarized Light Applications\n+\n+\n++\n+++\n++++\n++++\n++++\n++++\n++++\n++++\nAxiocam 202 mono\nAxiocam 305 mono\nAxiocam 506 mono\nAxiocam 702 mono\nAxiocam 705 mono\nAxiocam 712 mono\nAxiocam 807 mono\nAxiocam 820 mono\nHistology / Pathology\n+\n+++\n+++\n+\n+\n+\n+++\n+\nLive Cell Imaging\n+\n++\n++\n++++\n++++\n++++\n++++\n++++\nFluorescence Imaging\n+++\n++\n++\n++++\n++++\n++++\n++\n++++\nLow Light Imaging for Dim Samples\n++\n++\n++\n++++\n++++\n++++\n++\n++++\nSemiconductor Inspection\n++\n+++\n+++\n++\n++++\n++++\n+++\n++++\nLarge Samples\n+++\n+++\n+++\n+++\n+++\n++++\n+++\n++++\nMaterials Research\n++\n++++\n++++\n++\n++\n+++\n++++\n+++\nQuality Control\n+\n+++\n+++\n+\n+\n+\n+++\n+\nTeaching\n++++\n+++\n+++\n+\n++\n+\n+++\n+\nClinical Routine\n++++\n+++\n+++\n+\n+\n+\n+++\n+\nDynamic Range\n+++\n++++\n++++\n++++\n++++\n++++\n++++\n++++\nApplications\nMonochrome Cameras\nColor Cameras\n\n\nPage 20\nCarl Zeiss Microscopy GmbH\nCarl-Zeiss-Promenade 10\n07745 Jena, Germany\nEmail: microscopy@zeiss.com\nzeiss.com/axiocam\nNot all products are available in every country. Use of products for medical diagnostic, therapeutic or treatment purposes may be limited by local regulations. Contact your local ZEISS representative for more information.\nEN_40_010_025 | Version 3.2 | CZ 09-2022 | Design, scope of delivery and technical progress subject to change without notice. | © Carl Zeiss Microscopy GmbH\nFOR MORE INFORMATION/QUOTATION CONTACT:\nALLIED HIGH TECH PRODUCTS - EXCLUSIVE DISTRIBUTOR\n800-675-1118 (US/Canada)\ninfo@alliedhightech.com\nwww.alliedhightech.com\n